Functional training effects on postural control capacity in sports dancers / Efeitos do treinamento funcional sobre a capacidade de controle postural nos dançarinos esportivos / Efectos del entrenamiento funcional sobre la capacidad de control postural en los bailarines deportivos

ABSTRACT Introduction Functional training can improve athletes' coordination and physical control. Some studies prove the improvement of the strength of the core muscles after functional training. However, the research that questions athletes' dynamic balance submitted to functional training is inconclusive. Objective To analyze the methods of postural control and the impact of functional training on sports dancers. Methods Young sports dancers were randomly divided into control and experimental groups. Both groups performed traditional fitness training, and a functional training protocol was added to the experimental group. Mathematical statistics were employed in the comparative analysis of the fitness of the two groups of athletes before and after training. Results The athletes in the functional training group rapidly achieved postural control ability, with high statistical relevance(P<0.05). Conclusion Athletes need to adopt a scientific and systematic training plan when performing sports dance training. The establishment of the test indexes for postural control ability in athletes can be diversified. Evidence Level II; Therapeutic Studies - Investigating the results.

RESUMO Introdução O treinamento funcional pode melhorar a coordenação e o controle físico dos atletas. Também há estudos que comprovam a melhora da força dos músculos do core após o treinamento funcional. Porém ainda são inconclusivas as pesquisas que questionam o equilíbrio dinâmico dos atletas submetidos ao treino funcional. Objetivo Analisar os métodos de controle postural e o impacto do treinamento funcional em dançarinos esportivos. Métodos Jovens dançarinos esportivos foram divididos aleatoriamente em grupos controle e experimental. Os dois grupos executaram o treino de aptidão física tradicional e foi adicionado um protocolo de treino funcional ao grupo experimental. Estatísticas matemáticas foram empregadas na análise comparativa da aptidão física dos dois grupos de atletas, antes e depois do treinamento. Resultados Os atletas do grupo de treinamento funcional atingiram rapidamente a capacidade de controle postural, com alta relevância estatística(P<0,05). Conclusão Os atletas precisam adotar um plano de treinamento científico e sistemático ao realizarem treinos de dança esportiva. O estabelecimento dos índices de teste para a capacidade de controle postural nos atletas pode ser diversificado. Nível de evidência II; Estudos Terapêuticos - Investigação de Resultados.

RESUMEN Introducción El entrenamiento funcional puede mejorar la coordinación y el control físico de los atletas. También hay estudios que demuestran la mejora de la fuerza de los músculos del core tras el entrenamiento funcional. Sin embargo, las investigaciones que cuestionan el equilibrio dinámico de los atletas sometidos a un entrenamiento funcional aún no son concluyentes. Objetivo Analizar los métodos de control postural y el impacto del entrenamiento funcional en los bailarines deportivos. Métodos Los jóvenes bailarines deportivos fueron divididos aleatoriamente en grupos de control y experimentales. Ambos grupos realizaron un entrenamiento físico tradicional y se añadió un protocolo de entrenamiento funcional al grupo experimental. Se emplearon estadísticas matemáticas en el análisis comparativo de la forma física de los dos grupos de atletas antes y después del entrenamiento. Resultados Los atletas del grupo de entrenamiento funcional alcanzaron rápidamente la capacidad de control postural, con gran relevancia estadística (P<0,05). Conclusión Los atletas necesitan adoptar un plan de entrenamiento científico y sistemático cuando realizan un entrenamiento de danza deportiva. El establecimiento de los índices de prueba de la capacidad de control postural en los deportistas puede diversificarse. Nivel de evidencia II; Estudios terapéuticos - Investigación de resultados.

Mutation and Clinical Feature of IL-7R in Adult Patients with Acute Lymphoblastic Leukemia / 中国实验血液学杂志

OBJECTIVE@#To investigate the IL-7R gene mutation and clinical features of adult patients with acute lymphoblastic leukemia (ALL).@*METHODS@#One hundred sixty-four cases of newly treated adults with ALL from May 2016 to December 2018 were selected. Targeted and specific next-generation sequencing technology was used to detected a total of 16 types of Ph-like ALL mutations, which include IL-7R mutation, and the cilinical features, rate, types and sites of IL-7R were analyzed.@*RESULTS@#IL-7R mutation was determined in 10 cases of 164 adult patients with ALL and the total mutation frequency was 13 times (6.1%). Out of 10 cases 5 cases were male (50%), 5 cases were female (50%). 6 cases of B-ALL ( 60% ) and 4 cases of T-ALL (40%). The mutation site of all cases was located at exon 6, among which 6 cases had replacement mutations, 3 cases had deletion mutations and 4 cases had insertion mutations. In addition, 1 triple and 1 double mutation of IL-7R were found. Besides, six mutation sites were newly identified, including: c.720_724del, c.723_726del, c.721_722insAGTG, c.727_728insTAACGGCCCCCTGCT, c.727_728insATGCAGGGAGCGAA and c.728_729insAAGTGTCA.@*CONCLUSION@#Six novel mutation sites and a poor manifestation of IL-7R have been explored in this research. Thus more samples are required to study the effects of IL-7R mutation on ALL treatment.

Bipolarins A-H, eight new ophiobolin-type sesterterpenes with antimicrobial activity from fungus Bipolaris sp. TJ403-B1 / 中国天然药物

Bipolarins A-H (1-8), eight new tetracyclic ophiobolin-type sesterterpenes featuring a rare oxaspiro[4.4]nonane moiety, were isolated from cultures of fungus Bipolaris sp. TJ403-B1. Their structures and absolute configurations were elucidated by comprehensive spectroscopic analyses, single-crystal X-ray diffraction experiments, electronic circular dichroism and C NMR calculations. Additionally, compound 5 exhibited significant selective antimicrobial activity against Enterococcus faecalis with an MIC value 8 μg·mL.

Effect of ursolic acid combined with gemcitabine on proliferation and apoptosis of pancreatic cancer PANC-1 cells / 中国肿瘤生物治疗杂志

@#[Abstract] Objective: To study the effects of ursolic acid cooperated with gemcitabine on proliferation and apoptosis of pancreatic cancer PANC-1 cells. Methods: Human pancreatic cancer cell line PANC-1 was cultured in vitro with ursolic acid and gemcitabine respectively; and MTT assay was used to determine the IC50 of ursolic acid and gemcitabine, thus obtaining the best drug concentration. Ursolic acid (2 µmol/L) and gemcitabine (0.2 µmol/L) alone or in combination was used to treat PANC-1 cells; trypan blue assay was used to test cell viability, and PI staining was used to examine the cell apoptosis; wound healing was used to detect the proliferation and migration of PANC-1 cells. The protein expressions of P-JNK, Bcl-2, IL-6, P-Stat 3, NF-κB and Cox-2 in cells of each treatment group were detected using Western blotting. Results: Both ursolic acid and gemcitabine could significantly inhibit the proliferation of PANC-1 cells, and the IC50 is 13.67 and 2.78 µmol/L, respectively; and the final concentrations were determined at 2 and 0.2 µmol/L for ursolic acid and gemcitabine, respectively. Compared with single drug treatment, the combined treatment exerted a more prominent cell proliferation inhibition effect ([46.47±5.07]% vs [78.38±8.65]%, [76.12±3.23]%, all P<0.05), apoptosis-induction effect ([39.78± 7.01]% vs [20.35±8.51]%, [20.35±8.51]%, all P<0.01) and migration inhibition effect (P<0.01) on PANC-1 cells. Western blotting showed that the combined treatment strongly inhibited Bcl-2 and IL-6 expression, accelerated P-JNK protein expression compared with single drug treatment. Conclusion: The synergistic effect of ursolic acid and gemcitabine enhanced the inhibition on proliferation, migration, and promoted cell apoptosis of human pancreatic cancer cell line PANC-1, the mechanism may be associated with inhibition of Bcl-2, Il-6, P-stat 3 proteins and promotion of P-JNK protein.

Clinical analysis of elderly patients with osteoporotic hip fractures / 中华全科医师杂志

Clinical data of 448 patients aged ≥60 years with osteoporotic hip fractures admitted in Shanghai Huashan Hospital between January 2010 and December 2014 were retrospectively analyzed.Among 448 cases,there were 143 males and 305 females;196 cases aged 80-89 years (43.8％) and 137 cases aged 70-79 years (30.6％);304 cases of femoral neck fracture (67.9％) and 144 cases of intertrochanteric femoral fracture (32.1％);the fractures caused by fall in 328 cases (73.2％) and comorbidity with hypertension and diabetes mellitus in 415 cases (92.6％).Two hundred and sixty eight patients were followed up for 2 years,among whom re-fractures occurred in 17 cases.The study indicates that health education is important for prevention of osteoporotic fractures in elderly people.

Optimization of fermentation conditions for pristinamycin production by immobilized Streptomyces pristinaespiralis using response surface methodology

Response surface methodology was used to optimize the fermentation conditions for the production of pristinamycin by immobilization of Streptomyces pristinaespiralis F213 in shaking flask cultivation. Seed medium volume, fermentation medium volume and shaking speed of seed culture were found to have significant effects on pristinamycin production by the Plackett-Burman design. The steepest ascent method was adopted to approach the vicinity of optimum space, followed by central composite design for further optimization. A quadratic model was built to fit the pristinamycin production. The optimum conditions were found to be seed medium volume of 29.5 ml, fermentation medium volume of 28.8 ml, and shaking speed of seed culture at 204 rpm. At the optimum conditions, a production of 213 mg/l was obtained, which was in agreement with the maximum predicted pristinamycin yield of 209 mg/l. This is the first report on pristinamycins production by immobilized S. pristinaespiralis using response surface methodology.

Hereditary antithrombin deficiency and hyperhomocysteinemia in venous thromboembolic disease / 中国医学科学院学报

Hypercoagulability state is the result of the interplay of genetic predisposition and risk factors. Many key enzymes and reactions in coagulation and anti-coagulation system are involved. Hereditary antithrombin deficiency is one of the major risk factors of venous thromboembolic disease (VTE), whereas hyperhomocysteinemia may also play a role. This article reviews the recent researches on the contributions of these two factors to VTE.

Analysis of clinical characteristics of gastrointestinal cancer in Heilongjiang province, China 1998 to 2007 / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To provide basic information for epidemiological research of gastrointestinal (GI) malignant tumors.</p><p><b>METHODS</b>Data of GI cancer diagnosed in 15 hospitals of Heilongjiang province between January 1998 and December 2007 were analyzed retrospectively. The data mainly involved the age of onset, initial symptoms, pathological types, clinical staging and types of surgical procedure.</p><p><b>RESULTS</b>Gastric cancer was the most common type (45.8%) among the 33,540 GI cancer cases, then were rectal cancer (27.3%) and colon cancer (26.8%). Right colon cancer cases were more common than the left ones (1.3:1.0), particularly in people over 80 (2.1:1.0). Only 1.3% of colorectal cancer could be found in age under 30 years old. In patients aged 50 to 70, advanced gastric cancer accounted for 70.6%, advanced colon cancer 73.4% and advanced rectal cancer 72.4%. Well-moderately differentiated adenocarcinoma in early gastric cancer was 49.7%, early colon cancer 77.3% and rectal cancer 83.2%. Patients undergone radical excision in early gastric cancer accounted for 69.1%, advanced gastric cancer 79.9%, left colon cancer 91.9%, right colon cancer 83.9% and in rectal cancer for 88.3%.</p><p><b>CONCLUSIONS</b>People aged 50 to 70 tend to get GI cancer in Heilongjiang province. Gastric cancer is the most common GI cancer. Radical excision is the main choice of therapy.</p>

Expression,Purification and Antibody Preparation of Recombinant C8orf32 Protein / 中国生物工程杂志

C8orf32 is a gene which has not been functionally characterized,the mRNA level of this gene is significantly higher in breast cancer tissues than that in normal breast tissues.The amplified cDNA fragment was inserted into the pGEX-6P1 vector fused with the upstream GST gene.The expression vector was transformed into the E.coli BL21(DE3) strain and expression of GST-C8orf32 fusion protein was induced by IPTG..After removal of GST tag by site-specific protease,the C8orf32 protein fused with an eight amino acid peptide tag was obtained.The purity of recombinant C8orf32 protein was about 95%.The identity of the purified protein was confirmed by N-terminal sequencing and tandem mass spectrometry.The polyclonal antibody was prepared by immunizing the New Zealand white rabbits with C8orf32 protein.The polyclonal antibody was proved to recognize the C8orf32 protein correctly.The purified C8orf32 protein can be used for structural and functional studies and the polyclonal antibody can be used for tissue specific protein expression profiling.